ltbringer/blog

Reanimation

I am going through a course on edx.org named: Fundamentals of Neuroscience, Part 1: The Electrical Properties of the Neuron. Binge watching lecture series are not as effective as watching movie-series. To that extent I couldn't enjoy movie series at a stretch anyway. Interest alone can only take one so far. So I decided to take a break and watch a few treats hidden for the later parts of the course.

I remember visiting backyardbrains looking for neuroscience experiments and found a bunch of tools, blogs, experiments that I wish I had found before. I see a video lecture from the same team that makes these things.

This video session contained the following:

  1. Getting a Cockroach unconscious.
  2. Obtaining artifacts.
  3. Rate coding the neural activity.
  4. Getting the leg to move.

Prepare the subject

Cockroaches are cold blooded animals. So from the Nernst Equation:

E=RTzFlnion concentration outside ion concentration insideE = \frac{RT}{zF}\ln{\frac{\text{ion } \text{concentration } \text{outside }}{\text{ion } \text{concentration } \text{inside}}}

We can tell that as the temperature falls, the electric field due to any ions will cease to exist. This is all that's needed to put the cockroach unconscious, a bucket full of ice-water.

Obtaining artifacts

Credits - https://backyardbrains.com/experiments/spikerbox
Credits - https://backyardbrains.com/experiments/spikerbox

Once you have an unconscious cockroach, you umm... are required to do the thing.

Rate coding neural activity

Place the leg on cork surface and pin two electrodes in, as shown in the image:

Credits - https://backyardbrains.com/experiments/spikerbox
Credits - https://backyardbrains.com/experiments/spikerbox
We need a device to amplify the potential difference so that it can be seen while isolating noise. Touching the hairs of the leg causes neurons to spike and create a response that can be seen in the following image:

Credits - https://backyardbrains.com/experiments/spikerbox
Credits - https://backyardbrains.com/experiments/spikerbox

Zooming into a section shows the action potential of a neuron. The reason behind this behaviour is stated to be the opening and closing of Na+Na^{+}, K+{K}^{+} ion-channels.

Credits - https://backyardbrains.com/experiments/spikerbox
Credits - https://backyardbrains.com/experiments/spikerbox

Getting the leg to move

So far, we read the information that was going across the limb, reading the neural signals. Is it possible to send signals back? and what does that do?

The course shares that a song with high bass component is a good fit because of the lower frequency in the audio that gets encoded as electricity would cause the leg to twitch. The twitching is inversely proportional to the frequency and directly proportional to the volume. Here's the video from backyardbrains.

fin.